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KMID : 0545120120220050637
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 5 p.637 ~ p.641
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Biochemical Characterization of Thermophilic Dextranase from a Thermophilic Bacterium, Thermoanaerobacter pseudethanolicus
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Park Tae-Soon
Jeong Hyung-Jae
Ko Jin-A
Ryu Young-Bae
Park Su-Jin
Kim Do-Man
Kim Young-Min
Lee Woo-Song
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Abstract
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TPDex, a putative dextranase from Thermoanaerobacter pseudethanolicus, was purified as a single 70 kDa band of 7.37 U/mg. Its optimum pH was 5.2 and the enzyme was stable between pH 3.1 and 8.5 at 70oC. A half-life comparison showed that TPDex was stable for 7.4 h at 70oC, whereas Chaetominum dextranase (CEDex), currently used as a dextranase for sugar milling, was stable at 55oC. TPDex showed broad dextranase activity regardless of dextran types, including dextran T2000, 742CB dextran, and alternan. TPDex showed the highest thermostability among the characterized dextranases, and may be a suitable enzyme for use in sugar manufacture without decreased temperature.
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KEYWORD
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Thermostable, dextranase, Thermoanaereobacter, sugar processing, thermostability
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